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| Product Name: | Dog dopamine(DA) ELISA kit |
| Product Type: | ELISA Kit |
| Code : | CSB-EQ027496DO |
| Size : | 96T,5×96T,10×96T |
| Abbreviation: | DA |
| Species : | Dog |
| Sample Types: | serum, plasma and tissue homogenates |
| Detect Range: | 37.5 pg/ml-600 pg/ml |
| Sensitivity: | 37.5 pg/ml |
| Assay Time : | 1-5h |
| Sample Volume : | 50-100ul |
| Detection Wavelengt : | 450nm |
| Research Area: | Neuroscience |
| Protocol : |
Instructions Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
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| Precision : |
Intra-assay Precision (Precision within an assay): CV%<15 %Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<15% Three samples of known concentration were tested in twenty assays to assess. |
| Linearity : |
To assess the linearity of the assay, samples were spiked with high concentrations of dog DA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 92 Range % 90-97 1:2 Average % 95 Range % 88-100 1:4 Average % 95 Range % 89-100 1:8 Average % 92 Range % 86-99 |
| Recovery : |
The recovery of dog DA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 94 89-100 EDTA plasma (n=4) 95 85-105 |
| Typical Data: |
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average 600 0.296 0.294 0.295 300 0.524 0.532 0.528 150 0.905 0.901 0.903 75 1.724 1.763 1.744 37.5 2.543 2.588 2.566 |
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